Figure 4.

B cells from Traf5^−/− mice immunized with TNP-KLH/CFA show reduced expression of CD40-target genes. Expression levels of CD40-target genes, Fas and Lta, in B cells from WT and KO mice were evaluated using real-time RT-PCR. (A) Representative gating strategy for sorted B220⁺ B cell populations from the popliteal dLN cells of WT and KO mice. (B) Expression levels of Fas and Lta in sorted B220⁺ cells from the popliteal dLN cells of WT and KO mice, 4 days post-immunization. (C) Expression levels of Fas and Lta in sorted B220⁺ cells from unimmunized WT and KO mice. Data are presented relative to Rn18s expression as mean ± standard deviation (n = 3) from a representative experiment of two independent experiments showing similar results. * p < 0.05 (Student’s t-test).