Figure 6.

Angiogenic capability of DLS and HEP-DLS in vivo. (A) Gross appearances of scaffolds after 21 days of subcutaneous implantation in mice. Black circles indicate the neo-vessel formation within the scaffold (scale bar = 2 cm). (B) H&E staining showed more blood vessel formation (black arrows) in the HEP-DLS on 14 and 21 days post-implantation (scale bar = 100 μm); I, implanted areas; S, surrounding areas. (C) The number of blood vessels in each group of implants was expressed as the average per field on 14 and 21 days post-implantation (n = 4), * p < 0.05. (D) IHC staining of CD31 for the scaffolds on days 14 and 21 post-implantation, new blood vessels formed within the scaffolds (black arrows), (scale bar = 100 μm).