Figure 7.

Relevance of standard morphological properties and cell roundness feature for compound-induced MΦ (re-)polarization. (A) SImA ‘linear classifier’ analysis of indicated 24 h treated cpd- and biologically stimulated IDMs from one 384-well plate trained on IDM model 2 from Figure 5A. Model 2 differs in the usage of all or no standard morphology (area, roundness, width, length, and ratio of width to length) or no cell roundness features. DMSO-normalized %M2(-like)—%M1(-like) values correspond to a mean of 4 wells imaged at 20× magnification (5 fields per well). DMSO-normalized %M2(-like)—%M1(-like) values are listed in Table S6. (B,C) List of relevant features for M0, M1-like, and M2-like classification and their corresponding linear coefficient value based on the three models in A. The cell roundness feature is highlighted in orange. The common relevant features of all three ‘linear classifiers’ are illustrated in bold.