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The activity of NAD-IDH (A), AH (B), NAD-MDH (C), and FH (D) in the Y. lipolytica cells grown at pH of 4.0, 5.5, and 9.0. * A unit of enzymatic activity (EU) is calculated as the amount of enzyme needed for 1 μmol of the final product for 1 min at a temperature of 25 °C. Δ—statistically significant difference compared to the corresponding control, p < 0.005. Values are mean ± S.E.M from 5–6 independent experiments.
