FIG. 3.

Specificities of the antibodies raised to different regions of MSP4. Equivalent amounts (0.01 μg) of recombinant GST-MSP4A (lanes A), GST-MSP4B (lanes B), GST-MSP4C (lanes C), GST-MSP4D (lanes D), and GST alone were separated by SDS-PAGE, transferred to nitrocellulose, and then probed with the antibodies raised to GST-MSP4A (A), GST-MSP4B (B), GST-MSP4C (C), and GST-MSP4D (D). All of the antisera were preabsorbed against GST. Positions of molecular mass standards (kilodaltons) are given at the left in each panel.