FIG. 4.

Reactivity of an experimental antiserum to recombinant and parasite-derived MSP4. (A) Equivalent amounts (0.1 μg) of recombinant GST-MSP4A (lane A), GST-MSP4B (lane B), GST-MSP4C (lane C), GST-MSP4D (lane D), and GST alone were reduced and alkylated before being subjected to SDS-PAGE, transferred to nitrocellulose, and then probed with rabbit antibodies raised by a combination of priming with a DNA vaccine construct and boosting with MSP4E-His. (B) AA01 parasite extracts were either untreated (lane 1), reduced (lane 2), or reduced and alkylated (lane 3) before SDS-PAGE and transferred to nitrocellulose, and the blot was probed with the same antiserum. Positions of molecular mass standards (kilodaltons) are shown at the left in each panel.