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ELISA examining the reactivities of 22 patient sera to MSP4D either as a nonreduced (N) or as a reduced and alkylated (RA) antigen. The graph shows the ODs at 405 nm (OD405) of duplicate wells for each serum sample diluted to 1/500. Thirty control sera from areas where malaria is not endemic were tested at the same dilution, and the mean value + 3 standard deviations is defined as the cutoff point (shown as a horizontal line).
