FIG. 7.

Reduction-sensitive epitopes recognized by human sera on antigens of asexual-blood-stage parasites. (A) Reactivities of human sera to parasite proteins prepared under nonreducing (lane N) and reducing (lane R) conditions. (B) Reactivities of human sera to Triton X-114 phase-enriched parasite proteins under nonreducing (lane N) and reducing (lane R) conditions. The bands corresponding to MSP1, MSP2, MSP4, and MSP5 are indicated by arrows; the unidentified antigens at 38, 35, and 30 kDa with reduction-sensitive epitopes are indicated by asterisks. Positions of molecular mass standards (kilodaltons) are given in each panel.