Skip to main content
. 1999 May;67(5):2225–2232. doi: 10.1128/iai.67.5.2225-2232.1999

FIG. 4.

FIG. 4

Effect of recombinant Nramp1-cMyc protein expression on antibacterial activity of RAW264.7 macrophages. Nontransfected RAW264.7 cells (sample 1), Nramp1-cMyc-transfected RAW264.7 macrophage clone 13 (sample 2), clone 2.2 (sample 3), and a Nramp2-transfected RAW264.7 clone (sample 4) were seeded at 5 × 105 cells per well (5 wells per sample). Cells were infected with either S. typhimurium SL1344 (A) or the temperature-sensitive, replication-defective mutant TSΔ27 (B). After an initial 30-min phagocytosis period (T0), cell cultures were lysed at predetermined time intervals, and CFU counts were determined. The level of infection was determined by dividing the number of CFUt for each well at individual time points by the CFU0 (at T0) and is expressed as a percentage. The standard deviations for each time point are shown. The ranges in individual values for the samples at 24 h in panel A were as follows: sample 1, 4.4- to 3.0-fold increase; sample 2, 10 to 5%; sample 3, 81 to 57%; and sample 4, 4.2- to 3.1-fold increase. For panel B, the ranges for the samples at 24 h were as follows: sample 1, 11.5 to 8.5%; sample 2, 2.7 to 0.7%; sample 3, 1.6 to 0.3%; and sample 4, 4.2 to 2.1%.

HHS Vulnerability Disclosure