TABLE 1.
Gene | Primer
|
Internal hybridization probe
|
||
---|---|---|---|---|
Sequence | Expected size (bp) | Sequence | Size | |
Mammalian β-actin | 5′ AGC CAT CTC CTG CTC GAA GTC A 3′ | 450 | 5′ CTA CCT GAT GAA AAT CCT CAC TGA GCG TGG 3′ | 30 bases |
5′ ACC AAC TGG GAC GAC ATG GAG 3′ | ||||
T. parva β-actin | 5′ ACT GCT TTA GTT GTG GAC AAT GGT TCC GG 3′ | 1,112 | 5′ GTT CAT GCA GAA GAT TCT GGT TGA ACG TGG 3′ | 30 bases |
5′ GCA TTT ACG GTG CAC AAT ATT GGG GCC AC 3′ | ||||
T. parva hsp 70 | 5′ TGT AAC GAG AAG TTC AGA AGC 3′ | 998 | cDNA of hsp 70 | 1.8 kb |
5′ AGG GGC TCC GCC TGG GAA CCC 3′ |