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. 1999 May;67(5):2241–2249. doi: 10.1128/iai.67.5.2241-2249.1999

TABLE 1.

Oligonucleotide primer sequences employed in RT-PCR analysis of TpL treated with BW 720C

Gene Primer
Internal hybridization probe
Sequence Expected size (bp) Sequence Size
Mammalian β-actin 5′ AGC CAT CTC CTG CTC GAA GTC A 3′ 450 5′ CTA CCT GAT GAA AAT CCT CAC TGA GCG TGG 3′ 30 bases
5′ ACC AAC TGG GAC GAC ATG GAG 3′
T. parva β-actin 5′ ACT GCT TTA GTT GTG GAC AAT GGT TCC GG 3′ 1,112 5′ GTT CAT GCA GAA GAT TCT GGT TGA ACG TGG 3′ 30 bases
5′ GCA TTT ACG GTG CAC AAT ATT GGG GCC AC 3′
T. parva hsp 70 5′ TGT AAC GAG AAG TTC AGA AGC 3′ 998 cDNA of hsp 70 1.8 kb
5′ AGG GGC TCC GCC TGG GAA CCC 3′