TABLE 2.
Reactivity of WC1+ T cells freshly isolated from naive and immune cattle
| Animal no.a | SI for stimulator cellsb:
|
|||
|---|---|---|---|---|
| TpL | hsConA blasts | ConA blasts | Fixed TpL | |
| BK60 (i) | 15.4 | 6.7 | 0.6 | 5.5 |
| BJ244 (n) | 22.0 | 7.0 | 0.2 | 7.3 |
| BJ243 (i) | 36.3 | 9.9 | 0.0 | 8.6 |
| BL38 (in) | 40.1 | 18.3 | 0.9 | ND |
Cattle were either immune (i), naive (n), or undergoing infection (in) at the time of sampling. WC1+ T cells (104 cells/well) were FACS sorted from PBMC with MAb cc15 and cocultured with irradiated stimulator cells (104 cells/well) for 5 days in the presence of 10% TCGF.
Results are presented as SIs calculated as described in Materials and Methods. The SD of the mean was always <10% and is not given. Results are representative of three replicate experiments. Control counts per minute of freshly isolated WC1+ T cells cultured with 10% TCGF only were <2,300. Proliferative responses of the WC1+ T cells stimulated with TpL, fixed TpL, and hsConA blasts were shown to be significantly different from the proliferative responses of WC1+ T cells cocultured with ConA blasts (P < 0.05) by the Student one-tailed t test. ND, not determined.