TABLE 5.
Parasite specificity of the proliferative response of cloned and uncloned γδ T-cell linesa
Responder cell population | Result for stimulator cells
|
||
---|---|---|---|
TpL | hsConA blasts | ConA blasts | |
Line 6 | 6.8 | 0.9 | 0.6 |
clone F6 | 25.3 | 1.6 | 1.7 |
clone F15 | 35.8 | 2.1 | 1.9 |
clone A8 | 6.4 | 0.9 | 0.7 |
Results are shown as SIs calculated as described in Materials and Methods. The SD of the mean was always <10% and is not given. The results are representative of three separate experiments. The γδ T-cell line and clones (104 cells/well) were cultured for 5 days with irradiated stimulator cells (104 cells/well) in the presence of 10% TCGF. Control cultures of T cells cultured in the presence of 10% TCGF only showed <2,050 cpm. Proliferative responses of T cells cultured with TpL were shown to be significantly different from proliferative responses of T cells cultured with ConA blasts or hsConA blasts (P < 0.05) by the Student one-tailed t test.