TABLE 7.
The reactivity of an uncloned γδ T-cell line to TpL is MHC unrestricteda
| Animal source of stimulator cells | Result for stimulator cells:
|
|
|---|---|---|
| ConA | TpL | |
| BL38 | 0.9 | 9.7 |
| BL384 | 0.8 | 7.5 |
| BL383 | 0.7 | 5.8 |
| BL382 | 0.7 | 3.1 |
| BL380 | 0.7 | 6.4 |
| BL379 | 0.6 | 3.0 |
| BL378 | 1.1 | 7.9 |
| BL377 | 1.0 | 8.5 |
| BL376 | 0.9 | 5.1 |
| G169 | 2.1 | 14 |
| B641 | 2.2 | 6.5 |
| E98 | 1.9 | 12.4 |
a
Line 6 (104 cells/well) was stimulated with irradiated, autologous, and allogeneic TpL and the corresponding ConA blasts (104 cells/well) derived from randomly chosen animals. Shown are SIs calculated as described in Materials and Methods. The SD of the mean was always <10% and is not given. Control cultures of line 6 with 10% TCGF alone yielded <1,500 cpm. Proliferative responses of line 6 cultured in the presence of autologous or allogeneic TpL were shown to be significantly different from the proliferative responses of line 6 cocultured with ConA blasts (P < 0.05) by the Student one-tailed t test. Clones F6 and F8 responded similarly and are not shown.