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Fluorescent HPLC analyses of saturated oligosaccharide substrate chains reacted with rAly16-1. (A) Final products of 2AB-labeled M2~M6 substrate chains by rAly16-1; (B) time-course digestion of the 2AB-M5 chain by rAly16-1; (C) time-course digestion of 2AB-G5 and 2AB-G6 chains by rAly16-1; (D) time-course digestion of 2AB-M6 by rAly16-1. E (−), negative control group treated with the inactivated rAly16-1 enzyme.
