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. 2024 Nov 3;12(11):2228. doi: 10.3390/microorganisms12112228

Figure 1.

Figure 1

The examples of logarithmized melting curve fluorescence intensity values of the: (A) gluD gene amplification product obtained by real-time HRM-PCR using the LightCycler® 480 II apparatus (Roche, Rotkreuz, Switzerland) where gluD—lines showing fluorescence intensity obtained for glutamate dehydrogenase gene amplification; blue curves—melting charts of the specific gluD gene amplification product (positive results); red curves—melting of the unspecific amplification product (negative results); and (B) the examples of melting curves of the cdtB gene amplification product obtained by the real-time HRM-PCR method using the CFX Opus 96 Dx Real-Time PCR System thermal cycler where cdtB—binary toxin subunit B gene; green curves above the baseline—melting curves of the specific product of cdtB gene amplification (positive results); green curves below the baseline—melting curves of the non-specific amplification product (negative results).