FIG. 1.

Double immunofluorescence labeling of E. sennetsu, E. chaffeensis, HGE agent, and human TfR in THP-1 and HL-60 cells. Cells were infected with host cell-free ehrlichiae and harvested 3 (6 for E. sennetsu in HL-60 cells) days postinfection. Paired photomicrographs show FITC-labeled E. chaffeensis (E.C.), E. sennetsu (E.S.), or HGE agent (HGE) (white arrows) on the left and lissamine rhodamine-labeled human TfR on the right. Results are representative of three independent labeling experiments. Magnification, ×812.