Figure 2.

Analysis of tissue extract with online LLE DIP-MS from individual phases of three LLE methods (BD, BUME, and 3-PLE) in positive (left) and negative (right) modes. Bubble size represents the number of putatively annotated analytes for each class using an in-house-generated analyte list. The abbreviations of the classes are as follows: EAA, essential amino acids; OSM, other small metabolites; FA, fatty acyls, fatty acids, and fatty aldehydes; PC, phosphatidylcholines; LPC, lysophosphatidylcholines; PE, phosphatidylethanolamines; LPE, lysophosphatidylethanolamines; PS, phosphatidylserines; LPS, lipopolysaccharides; MG, monoacylglycerols; DG, diacylglycerols; SM, sphingomyelins; SPB, sphingoid bases; Steroids, sterols and sterol lipids; Cer, ceramides; SHexCer, sulfatide species; PI, phosphatidylinositols; LPI, lysophosphatidylinositols; PA, phosphatidic acids; LPA, lysophosphatidic acids; PG, phosphatidylglycerols; and LPG, lysophosphatidylglycerols.