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Fluorescence imaging of A549-RFP cells in the main channel and module of the MiFD. (a-1) Images of the main channel, yellow rectangle: segment for magnification in (a-2). (a-2) Images of cells in higher magnification. (b) Images of spheroids in an MiFD module, yellow arrows: indicating spheroid position. Background subtraction function applied with Fiji/ImageJ, original image in Figure A5.
