FIG. 4.

Amino acid sequences representing nine IgE binding epitopes of Asp f 2. The continuous linear epitopes were identified by synthesizing overlapping peptides spanning the complete protein. The length of the epitopes varied from 3 (HWR; epitope 3) to 7 (ATQRRQI; epitope 1) aa. Various amino acids in epitope 4 are shown at the right as an example of how the epitope was derived by synthesizing various overlapping peptides.