FIG. 5.

(A) Specificity of IgE binding of 3-aa-long peptides of Asp f 2, synthesized at an offset of 1 aa encompassing epitope 3 HWR (spot 5). Open-lettered amino acids are the part of the selected epitopes. (B) IgE antibody binding of 5-aa-long peptides synthesized at an offset of 1 aa; spot 6 represents the selected epitope 2 (RKYFG) of Asp f 2. (C) IgE binding of overlapping peptides encompassing epitope 4 (YTTRR) of Asp f 2. All peptides were evaluated for IgE binding by using pooled ABPA sera at a dilution of 1:10. The left panel represents densitometric scanning of the spots as a measure of intensity of IgE-peptide reaction (for details, see Materials and Methods).