FIG. 7.

IgE antibody binding of sera from patients with ABPA (n = 24; □), allergic asthma (n = 10; ○) and from healthy controls (n = 10; ▵) to purified recombinant Asp f 2, Asp f 2A (aa 1 to 203), Asp f 2B (aa 68 to 203), and Asp f 2C (aa 68 to 268). The cutoff level for positive reaction (mean optical density [O.D.] + 2 standard deviations of the control values) is indicated by the solid line. The statistical significance of IgE antibody binding of the recombinant Asp f 2 and the fragments with sera from ABPA in comparison to healthy controls are as follows: Asp f 2, P < 0.008; Asp f 2A, P < 0.05); Asp f 2B, P < 0.116); and Asp f 2C, P < 0.06.