Figure 2.

rSAG2A alters the pro-inflammatory and anti-inflammatory cytokines expression in PECs infected with T. gondii. Cytokine transcript accumulation was evaluated during the presence of parasites for 3 h in PECs from both BALB/c and C57BL/6 mice or treated with rSAG2A during 24 h, through qPCR. (A) INOS, (B) Arginase, (C) TNF-α, (D) IL-10, (E) IL-1β, (F) TGF-β, and (G) IL-6. (H) Hierarchical clustering for the same mean relative quantification (RQ) values for the same genes, represented in z score scale, in the different conditions studied. Data represent mean + SD of two independent experiments using a pool of PECs n = 4 animals/experiment. * p < 0.05, ** p < 0.01, and *** p < 0.001 between control and rSAG2A of 6 replicates/group by ANOVA followed by the Tukey post-test.