Table 2.

The experimental design summary shows the different factors, runs, and the associated response (LNP size average, nm). The factors are x₁ (initial lignin concentration, g/L), x₂ (solvent flow rate, ml/min), x₃ (antisolvent composition, water/EtOH, v/v), x₄ (antisolvent ratio, solvent/antisolvent, v/v), and x₅ (antisolvent stirring speed, rpm).

Run	Design Factors (25−1)					Design Response
	x1	x2	x3	x4	x5	LNPs Size Average
1	10	100	80	5	150	323 ± 6
2	20	2	100	5	1000	112 ± 3
3	10	100	80	20	1000	109 ± 2
4	20	2	100	20	150	114 ± 4
5	20	100	80	5	1000	266 ± 2
6	10	2	100	5	150	128 ± 3
7	20	100	80	20	150	173 ± 3
8	10	2	100	20	1000	50 ± 2
9	20	2	80	5	150	340 ± 6
10	10	100	100	5	1000	87 ± 3
11	20	2	80	20	1000	127 ± 3
12	10	100	100	20	150	62 ± 2
13	10	2	80	5	1000	237 ± 5
14	20	100	100	5	150	134 ± 3
15	10	2	80	20	150	153 ± 3
16	20	100	100	20	1000	73 ± 2
17	15	51	90	12.5	575	132 ± 2