Figure 6.

Live-cell imaging of peripheral NK cytotoxicity treated with Sig-7/9 NPs, Control NPs and non-treated peripheral NK cytotoxicity on MCF-7 cells. MCF-7 cells labeled with CMFDA green co-cultured with primary NK labeled with CMTMR orange. Dead cells shown in red. (A) Representative confocal images taken at different time points (0, 4 and 8 h). Scale bar = 100 µm. Orange arrows are pointing at the pNK cells forming immunological synapses with the target cells. (B) The percentages of killed MCF-7 cells by the treated and non-treated peripheral NK. Error bars represent SEM of three independent experiments. Statistical significance was determined using two-way ANOVA. (*) p value ≤ 0.05.