Figure 3.

Effect of PDREP on MAPKs and NF-κB activation in SW1353 cells. SW1353 cells were cultured with PDREP (0, 30, 100, or 300 µg/mL) or MSM (300 µg/mL) and then treated with IL-1β (10 ng/mL) for 24 h. (A) Protein expression levels were analyzed by Western blotting using anti-ERK, p-ERK, p38, p-p38, JNK, p-JNK, NF-κB, p-NF-κB, and iNOS antibodies. (B–F) Band intensities were quantified using ImageJ software. Values are presented as mean ± SD (n = 3). Bars labeled with different superscript letters indicate p < 0.05.