Figure 3.

Chelidonine and tetrandrine inhibited B16F10 cells and tumor growth. (A) shows that cells were treated with different concentrations of CHD and TED. Fixed 8 μmol/L of TED and different concentrations of CHD (0, 1.4, 2.8, 5.6, 11.2 μmol/L) on B16F10 cells for 24 h. (B) shows 8 μmol/L TED, and 5.6 μmol/L on B16F10 cells. (C) shows the melanin content on the B16F10 cell was detected. (D) shows the treatment in the mice’s body after inoculating the tumor. Experiments were divided into three groups: the model group, the individually administered group with different concentrations of CHD, and the co-treatment group. (E–G) show that the tumor was removed from the mice’s bodies with varying doses of the two compounds, and tumor volume and size were measured. ** p < 0.01 vs. control group, *** p < 0.001 vs. control group.