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. 2024 Nov 16;29(22):5412. doi: 10.3390/molecules29225412

Figure 6.

Figure 6

Apoptosis induction and cell cycle arrest on B16F10 cells. (A) Annexin V-PI-staining analysis was performed to evaluate apoptotic cell death in B16F10 cells after CHD, TED, and co-treatment. (B) PI-staining analysis was performed to evaluate the cell cycle change in B16F10 cells after CHD, TED, and co-treatment. (C) qPCR products showed the effects of CHE, TED, and co-treatment on mRNA expression of p53, p21, cyclin D1, cdk2, bax, and bcl-2 against gapdh. (D) Western blot analysis of indicated proteins in CHD, TED, and co-treatment group. * p < 0.05 vs. control group, ** p < 0.01 vs. control group, *** p < 0.001 vs. control group.