Figure 9.

Effect of PIC-OCT on microglial migration and poly (ADP-ribose) polymerase-1 (PARP1) expression. Rd10 mice were intravitreally (IVT) injected with 10 mM PIC-OCT or vehicle. At P28, the mice were sacrificed, and retinal sections were immunostained with anti-glial fibrillary acidic protein (GFAP) (A,B; green), anti-ionized calcium-binding adaptor molecule 1 (Iba-1) (A’,B’; red), and DAPI (A”,B”; blue). Additionally, other retinal sections were prepared for immunocytochemistry using PARP1 and detected with diaminobenzidine (DAB) (C,D). RPE: retinal pigment epithelium; IS/OS: inner segment/outer segment; ONL: outer nuclear layer; INL: inner nuclear layer; GCL: ganglion cell layer. The arrows indicate the ONL thickness (A”,B”,C,D). Scale bars represent 25 μm.