Table 1.
Retention times, MRM transitions, limit of detection (LOD), and limit of quantification (LOQ) for α-TOH and its long-chain metabolites in human serum.
| Compounds | Retention Time (min) | Ion Species | Qualifier | Quantifier | LOD/LOQ a nmol/L | Accuracy Recovery (rel.) |
Precision RSDr |
|---|---|---|---|---|---|---|---|
| α-13′-COOH | 10.4 | [M+H]+ | 461.30 | 165.00/189.00/414.00 | 5.3/17.8 | ||
| d6-α-13′-COOH | 10.4 | [M+H]+ | 467.30 | 171.00/194.00/420.00 | 89% | 2.0% | |
| α-13′-OH | 11.0 | [M+H]+ | 447.30 | 165.00/429.40 | 3.4/11.2 | ||
| d6-α-13′-OH | 11.0 | [M+H]+ | 453.30 | 171.00/435.40 | 94% | 6.1% | |
| α-TOH | 14.8 | [M+H]+ | 431.30 431.30 → 165.00 |
165.00 137.00 |
3490/11,635 | ||
| d6-α-TOH | 14.8 | [M+H]+ | 437.30 437.30 → 171.00 |
171.00 143.00 |
88% | 12.6% |
a instrumental LOD/LOQ, calculated with calibration data of analyte/ILS area ratios and analyte concentration.