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. 2024 Nov 20;29(22):5476. doi: 10.3390/molecules29225476

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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HPLC profiles of the peptide substrates treated with the CCN′ mutant proteins. The peptide fragments were analyzed by amino acid analysis. Their identified sequences are indicated in the profiles. (A) The CCN′ protein was derived from [K8D]-proCCN′. (B–G) represent [D187E]-, [D187S]-, [D187A]-, [D187N]-, [S188A]-, and [S188D]-CCN′. (H) The peptide substrates were allowed to stand for 3 days at 37 °C without the mutant proteins. There were no significant digests in the absence of the mutant enzymes. Asterisks (*) indicate impurities derived from the LAAFGLF peptide.

HPLC profiles of the peptide substrates treated with the CCN′ mutant proteins. The peptide fragments were analyzed by amino acid analysis. Their identified sequences are indicated in the profiles. (A) The CCN′ protein was derived from [K8D]-proCCN′. (B–G) represent [D187E]-, [D187S]-, [D187A]-, [D187N]-, [S188A]-, and [S188D]-CCN′. (H) The peptide substrates were allowed to stand for 3 days at 37 °C without the mutant proteins. There were no significant digests in the absence of the mutant enzymes. Asterisks (*) indicate impurities derived from the LAAFGLF peptide.