Figure 5.

The anti-tumor activity of AXL inhibitor plus WIN55212-2 treatment in the HCT-8 cell xenograft mice. BALB/c nude mice were injected subcutaneously in the flank with HCT-8 colon cancer pieces and intratumorally treated with TP-0903 (3.85 μg/kg or 0.13 mg/kg) alone or in combination with WIN55212-2 (2.5 mg/kg) every two days. Cisplatin was used as a control (5 mg/kg per week). The tumor photograph (A), tumor volume (B) and tumor mass (C) of individual mice were recorded. (D) The apoptosis of tumor sections was determined by a colorimetric TUNEL assay. (E) The TUNEL-positive rate was calculated and shown in the histogram. (F) The microvessel density of tumor tissue was evaluated by immunostaining. (G) The percentage of CD31-positive cells was examined and is shown in the histogram. * p < 0.05, ** p < 0.01, *** p < 0.001 indicate a significant difference.