FIG. 3.

Kinetics of ASPND1 and AgMb production with time. A. nidulans (10⁵ conidia/ml) was cultured at 28°C in CD medium. At the indicated times, samples were withdrawn to determine mycelial dry weight (triangles) and to obtain total mycelial extracts, which were separated on an SDS–14% polyacrylamide gel (20 μg of protein per lane) and immunoblotted by using as the first antibody anti-ASPND1 (insert, ASPND1) or antimembrane (insert, AgMb) rabbit serum at 1/500 dilution. ASPND1 and AgMb bands were quantified by densitometry, and the IOD values obtained were plotted (on a semilogarithmic scale) against time. AgMb was constitutively produced (black circles), whereas the synthesis of ASPND1 (white circles) increased with time in parallel with the increase in mycelial dry weight (white triangles). Results are means (± standard deviations) of three separate determinations.