FIG. 5.

Effect of the addition of increasing concentrations of Zn²⁺ or of 50 μM divalent ions on ASPND1 production. Several parallel cultures of A. nidulans (10⁶ conidia/ml) were grown at 28°C for 12 h. Each of the cultures was then supplemented with ZnSO₄ to obtain final concentrations of 0, 0.1, 0.5, 1.0, 5.0, and 10.0 μM Zn²⁺ (A and C) or with the indicated divalent metal salts (sulfate form) to achieve a final concentration of 50 μM (B and D). The amount of immunoreactive ASPND1 or AgMb present in total mycelial extracts obtained from all cultures after a further 12 h of growth was determined as described in the text by densitometry of the corresponding immunoblots (A and B). Results in panel A are expressed as the percentage of the maximal IOD measured for ASPND1 or AgMb; results in panel B are expressed as the percentage of the maximal IOD ratio between ASPND1 and AgMb. The amount of total protein in cell extracts (C and D) was determined as indicated in Materials and Methods. Results are means (± standard deviations) of three separate determinations. Ctrl, control.