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. 2024 Nov 4;16(11):477. doi: 10.3390/toxins16110477

Figure 2.

Figure 2

Effects of culture conditions on Lac-W-AS-mediated ZEN degradation. (A) Effect of pH on ZEN degradation. (B) Effect of temperature on ZEN degradation. (C) Effect of concentration of Lac-W and AS on ZEN degradation. (D) Effect of ions on ZEN degradation. The Lac-W was incubated with different ions at room temperature for 10 min, followed by ZEN. Reaction conditions: initial ZEN 1 µg/mL, culture conditions in static condition, pH 2.0–9.0, 20–80 °C, concentration of Lac-W or AS were 0.1–1 U/mL or 0.1–10 mM, and incubation time 1 h. The temperature, Lac-W/AS concentration, and metal ion assays were performed under optimal conditions derived from the pH, temperature, and Lac-W/AS reactions, respectively. Three independent replicates were performed for each assay. Each reaction was repeated three times.