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. 2024 Nov 4;16(11):477. doi: 10.3390/toxins16110477

Figure 4.

Figure 4

Mass spectra analysis of the ZEN degradation by Lac-W-AS. (A) Total ion chromatogram of ZEN (retention time of 5.16 min). (B) Total ion chromatogram of the ZEN degradation product (retention time of 3.62 min). (C) The mass spectra analysis of ZEN (C18H22O5; [M-H]− with m/z 317.2715). (D) The mass spectra analysis of the ZEN degradation product by Lac-W (C18H22O6; [M-H]− with m/z 333.1376). Mass spectrum parameters: ESI ion source, negative ion mode, and full scanning range 100–1500 m/z. The ion transfer tube temperature was set to 325 °C, and the vaporizer temperature was 350 °C. Orbitrap had a resolution of 120,000. The metabolite analysis software was Compound Discovery 3.0.