Figure 2.

Fine mapping of RppSLN. (a) The resistant inbred line N531_R and susceptible inbred lines F35 and hybrid F₁ (N531_R×F35). Scale bar: 30 cm. (b) A major southern corn rust-related QTL located in the F₂ population of N531_R/F35. LOD, logarithm of odds. (c) A large-scale isolation of 2253 F₂ individual plants combined with a genotyping linkage analysis delimited RppSLN to a 38 kb interval flanked by the markers W4 and W6. The RppSLN region contains two putative NLR genes, which encode NBS-LRR proteins. Green and yellow segments represent N531_R and F35 alleles, respectively. Phenotypes are provided next to the haplotype. R, resistant; S, susceptible.