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AHB venom toxins are strong C5a anaphylatoxin inducers. C5a anaphylatoxin was detected in NHS samples incubated with AmV amounts varying of 5-1000 µg/mL (A–I) by using CBA and evaluated statistically by two-way ANOVA followed by Sidak’s multiple comparison test in GraphPad PRISM 8. p ≤ 0.05 was considered to indicate statistical significance. # T0/NHS x all times; * AmV x Vehicle; α 30 min x 60 or 120 min; β 60 min x 30 or 120 min; δ 120 min x 30 or 60 min.
