Figure 2.

Innate immune response elicited in muscle and DLNs from mice immunized with a DNA vector expressing the polyvalent multiepitopic CoV2-TMEP protein. (A) Immunization schedule. Mice were inoculated with DNA-CoV2-TMEP or PBS by intramuscular (i.m.) route. At the indicated days post-inoculation, total muscle from the site of inoculation and DLNs were harvested and processed as described in the Materials and Methods section. (B,C) Immune cell populations present in muscle (B) and DLNs (C) determined by flow cytometry. Data are shown as mean and SD. BCs: B cells; TCs: T cells; NKs: natural killer cells; moCs: monocyte-derived cells; cDCs: conventional dendritic cells; NOs: neutrophils. *, p < 0.05; **, p < 0.005.