Figure 2.
Amplification and sequencing approach for ASFV. (A) Schematic overview of workflow established in this study (Created in BioRender. Licheri, M. (2024) BioRender.com/b70m288); (B) the sequences obtained with three ASFV samples (EDTA85, 87 and 88) amplified with or without pre-treatment were used to generate coverage plots showing the sequencing depth (log10 scale, y-axis) in the function of each nucleotide position (x-axis); (C) the percentages of the obtained mapped reads were compared between the three different pre-treatment conditions (no, heat, or alkaline pre-treatment; ns: not significant, *: p ≤ 0.05, ***: p ≤ 0.001, paired t-test).