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Sensitivity of the SWGA MDA combined with the alkaline denaturation. (A) Four 10-fold serial dilutions of ASFV (on the bottom is the corresponding Ct-value, NC = negative control) were amplified in independent replicates using the developed approach, resulting in similar 20× coverage up to a Ct-value of 23.1; (B) the corresponding mean sequencing depth of the four 10-fold serial dilutions shows a marked reduction after the second 10-fold serial dilution.
