Figure 2.

Phylogenetic tree based on partial nucleotide sequences of the recA gene (730 bp) of the LMN08 strain and previously reported Burkholderia strains. The sequence alignment and construction of the phylogenetic tree were performed using MEGA version 10 software [14]. A phylogenetic tree was reconstructed using the maximum-likelihood method with 1000 bootstrap replicates. The fittest substitution model for the data set was selected based on the Bayesian information criterion score. The model used in this study was the Tamura 3-parameter incorporating a gamma distribution and invariant sites (T92+G+I). The scale bar indicates the number of nucleotide substitutions per site, and bootstrap values (1000 replicates) above 60 are shown. The strain name and GenBank accession number are shown next to the species names. The background of B. pseudomultivorans is highlighted in light blue.