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Construction and quality assessment of the cDNA library. (A) Electrophoretic analysis of total RNA extracted from chicken kidney tissue. Lane M represents the DL5000 DNA marker, while lane 1 shows the total RNA. (B) Identification of the inserted DNAs from the cDNA library. Lane M corresponds to the DL5000 DNA marker, and lanes 1−32 display 32 individual recombinant colonies that were randomly selected and amplified by PCR using universal primers of the pGADT7-Rec vector.
