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. 2024 Oct 31;11(11):531. doi: 10.3390/vetsci11110531

Figure 7.

Figure 7

IBV infection upregulates DNAJA1 expression to promote viral replication. (A) The mRNA expression levels of potential host proteins interacting with Nsp2 were detected using qRT-PCR 24 h post IBV infection. Experimental data were pooled and analyzed using t-tests. CEK cells were transfected with pcDNA3.1-DANAJ1-Myc for 24 h and then infected with IBV; the cells were collected 12, 24, 36, and 48 h later. qRT-PCR was used to detect DNAJA1 (B) and IBV-N (C) mRNA expression levels, while Western Blotting was employed to detect the expression of indicated proteins (D). Additionally, CEK cells were transfected with siRNA to silence DNAJA1 for 24 h and then infected with IBV. Cells were collected 12, 24, 36, and 48 h post-inoculation. qRT-PCR was used to measure DNAJA1 (E) and viral N protein (F) mRNA expression levels, and Western Blotting was used to analyze protein expression (G). The data are presented as the mean ± SD. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001.