Table 1.

Antibody information and retrieval conditions.

Primary Antibody Name	Antigen Retrieval Conditions	Primary Antibody Host Species	Secondary Antibody Name
S100A4	Citrate buffer (pH 6.0), microwave treatment: medium heat for 10 min, pause for 5 min, followed by medium–low heat for 5 min, pause for 2 min, and another round of medium–low heat for 5 min	Rabbit	HRP-conjugated Goat Anti-Rabbit IgG
CDK4	EDTA buffer (pH 9.0), microwave: medium heat 10 min, pause 5 min, medium–low heat 5 min, pause 2 min, medium–low heat 5 min	Rabbit	HRP-conjugated Goat Anti-Rabbit IgG
MDM2	EDTA buffer (pH 8.0), water bath at 90 °C for 30 min	Rabbit	HRP-conjugated Goat Anti-Rabbit IgG
CD34	Citrate buffer (pH 6.0), microwave: medium heat 10 min, pause 5 min, medium–low heat 5 min, pause 2 min, medium–low heat 5 min	Rabbit	HRP-conjugated Goat Anti-Rabbit IgG
Vimentin	EDTA buffer (pH 8.0), water bath at 90 °C for 30 min	Rabbit	HRP-conjugated Goat Anti-Rabbit IgG
Leptin	Citrate buffer (pH 6.0), microwave: medium heat 10 min, pause 5 min, medium–low heat 5 min, pause 2 min, medium–low heat 5 min	Rabbit	HRP-conjugated Goat Anti-Rabbit IgG