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. 2024 Oct 29;16(11):1687. doi: 10.3390/v16111687

Figure 2.

Figure 2

Isolation of PRRSV and PCV2 strains; (A) The PRRSV strain was isolated in PAM cells, resulting in significant Cytopathic effects (72 hpi 100×); (B) The PRRSV strain was isolated in Marc-145 cells, resulting in significant Cytopathic effects (72 hpi 200×); (C) Isolation of PRRSV in Marc-145 cells: observations of lesions in Marc-145 cells post-infection and immunofluorescence assay (IFA) detection using pig-derived PRRSV-positive serum (72 hpi 100×); all isolated PRRSV strains induced similar results; (D) Isolation of porcine circovirus type 2 (PCV2) in PK-15 cells: observations of lesions in PK-15 cells post-infection and immunofluorescence assay (IFA) detection using PCV2-positive serum sourced from pigs (72 hpi 200×).