PRRSV GP5 protein amino acid variation analysis, with the red box indicating the sequences of the strains obtained in this study. The black box area from left to right represents the signal peptide region, two hypervariable regions (HVRs), and three transmembrane regions (TMs). The gray area from left to right represents bait epitope region, primary neutralizing antigenic epitope region (PNE), two T-cell epitope regions, and one B-cell epitope region. The orange area indicates distinguishing sites on the GP5 protein for identifying PRRSV vaccine strains, field strains, and variations in virulence.