Extended Data Fig. 5. Involvement of mTOR pathway in inflammatory response of BMDM under different oxygen tension.
a. Phosphorylation status of p70 S6K was examined to evaluate mTOR pathway activity. LPS treatment increased p70 S6K phosphorylation (P-p70 S6K), which was decreased by mTOR inhibitor rapamycin regardless of the oxygen tension. Norm: BMDMs differentiated and stimulated with LPS under normoxia, AHyp: BMDMs differentiated under normoxia and stimulated with LPS under 1% O2, CHyp: BMDMs differentiated and stimulated with LPS under 1% O2. A representative result from 2 independent experiments is shown. b. Gene expression in BMDMs after LPS stimulation (n = 3). Norm, AHyp, and CHyp BMDMs treated with or without rapamycin were examined. Error bars represent S.E.M. of 3 biological replicates.