Extended Data Fig. 9. Supplementation of pyridoxal and supersulfide donor, GSSSG, to CHyp-BMDM.
a. Expression of cytokine genes in BMDMs after LPS stimulation (n = 3). Norm: BMDMs differentiated and stimulated with LPS under normoxia, CHyp: BMDMs differentiated and stimulated with LPS under 1% O2, CHyp + Pyridoxal: BMDMs differentiated under 1% oxygen in the presence of 50 μg/ml pyridoxal. b. Expression of cytokine genes in BMDMs after LPS stimulation with or without PNPO overexpression (n = 3). GFP was overexpression as a negative control. c. SSP4 staining to detect supersulfides in BMDM. Representative SSP4 staining (left) and its quantification (right). Scale bars correspond to 50 μm. d. Expression of cytokine genes in BMDMs treated with a supersulfide donor, GSSSG, and oxidized glutathione, GSSG, as a negative control (n = 3). A representative result from 3 independent experiments is shown. Error bars represent S.E.M. (a, b, d). Two-way ANOVA (a, b, d) and two-sided Student’s t-test (c) were conducted to evaluate statistical significance.