Extended Data Fig. 5. Striatal Glutathione levels and redox status are impaired in Trkb^Penk-KO mutants.

a, b) Concentration of total (tGSH) and reduced (GSH) glutathione in striatal tissues of Trkb^Penk-KO and control littermates at 3 M and 8 M of age. The measurement is expressed as nmol/mg protein and presented as a fold change. a) Data are presented as box plots min-max (median), cytosolic fraction at 3 M, tGSH (Trkb^Penk-WT 1.0 ± 0.03; Trkb^Penk-KO, 0.81 ± 0.06, p = 0.02); GSH (Trkb^Penk-WT, 1:00 ± 0.05; Trkb^Penk-KO 0.78 ± 0.06, p = 0.02); GSSG (Trkb^Penk-WT, 1:00 ± 0.08; Trkb^Penk-KO, 1.54 ± 0.48, p = 0.29) and GSH:GSSG (Trkb^Penk-WT, 1:00 ± 0.09; Trkb^Penk-KO, 0.71 ± 0.15, p = 0.13). b) Whole homogenate at 3 M, tGSH (Trkb^Penk-WT, 1:00 ± 0.06; Trkb^Penk-KO, 0.89 ± 0.01, p = 0.16); GSH (Trkb^Penk-WT, 1:00 ± 0.10; Trkb^Penk-KO, 0.74 ± 0.14, p = 0.14); GSSG (Trkb^Penk-WT, 1:00 ± 0.09; Trkb^Penk-KO, 1.48 ± 0.50, p = 0.50) and GSH:GSSG (Trkb^Penk-WT, 1:00 ± 0.20; Trkb^Penk-KO, 0.76 ± 0.43, p = 0.63). c) Data are presented as box plots min-max (median), mitochondrial fraction at 3 M, tGSH (Trkb^Penk-WT, 1:00 ± 0.07; Trkb^Penk-KO, 0.83 ± 0.05, p = 0.07); GSH (Trkb^Penk-WT, 1:00 ± 0.07; Trkb^Penk-KO, 0.84 ± 0.05, p = 0.09); GSSG (Trkb^Penk-WT, 1:00 ± 0.10; Trkb^Penk-KO, 0.78 ± 0.10, p = 0.15) and GSH:GSSG (Trkb^Penk-WT, 1:00 ± 0.11; Trkb^Penk-KO, 1.12 ± 0.16, p = 0.55). d) Whole homogenate at 8 M, data are presented as box plots min-max (median), tGSH (Trkb^Penk-WT, 1:00 ± 0.03; Trkb^Penk-KO, 0.82 ± 0.03, p = 0.003); GSH (Trkb^Penk-WT, 1:00 ± 0.05; Trkb^Penk-KO, 0.77 ± 0.07, p = 0.02); GSSG (Trkb^Penk-WT, 1:00 ± 0.26; Trkb^Penk-KO, 1.06 ± 0.25, p = 0.87) and GSH:GSSG (Trkb^Penk-WT, 1:00 ± 0.23; Trkb^Penk-KO, 0.77 ± 0.23, p = 0.50). Values are means ± SEM; p statistic from unpaired, two-tailed, Student’s t-test. Animals, a, c, d) n = 6 each genotype (a, c, female mice; d, male mice. b) n = 3 each genotype (male mice). Box plot statistics for panels c, e, and f are reported in the source data.

Source data