Table 2.
Primers used in the present study.
| Target Genes | Primer Sequences (5′-3′) | GenBank Accession | |
|---|---|---|---|
| Plasmid construction | |||
| miR-130b | F:GATCCGCCTGCCTGACACTCTTTCCCTGTTGCACTACTGTGGGCCACTGGGAAGCAGTGCAATGATGAAAGGGCATCAGTCAGGCTTTTTTGGAAA | R:AGCTTTTCCAAAAAAGCCTGACTGATGCCCTTTCATCATTGCACTGCTTCCCAGTGGCCCACAGTAGTGCAACAGGGAAAGAGTGTCAGGCAGGCG | MI0013136 |
| miR-SC | F:GATCCGACTTACAGCCAGTTCCTAGTATAGTGAAGCAGCAGATGGTATACTAGGAACTGGCTGTAAGCTTTTTTTGGAAA | R:AGCTTTTCCAAAAAAAGCTTACAGCCAGTTCCTAGTATACCATCTGCTGCTTCACTATACTAGGAACTGGCTGTAAGTCG | ENSMUST00000115567.2 |
| miR-130b expression | |||
| miR-130b | CAGUGCAAUGAUGAAAGGGCAU | TAGAGTGAGTGTAGCGAGCA | MIMAT0013922 |
| Exogenous reference |
GTGACCCACGATGTGTATTCGC | GTGACCCACGATGTGTATTCGC | |
| poly(T) adapter | TAGAGTGAGTGTAGCGAGCACAGAATTAATACGACTCACTATAGGTTTTTTTTTTTTTTTTVN | ||
| mRNA expression | |||
| TNF-α | F: ACCACCATCAAGGACTCA | R: AGGTCTGAAGGTAGGAAGG | NM_001278601.1 |
| IL-1α | F: AAGAAGAGACGGCTGAGT | R: GTGGTGCTGAGATAGTGTT | NM_010554.4 |
| IL-1β | F: CTTCAGGCAGGCAGTATC | R: CAGCAGGTTATCATCATCATC | NM_008361.4 |
| IL-6 | F: CCCCAATTTCCAATGCTCTCC | R: CGCACTAGGTTTGCCGAGTA | NM_031168.2 |
| IL-10 | F:CAGTACAGCCGGGAAGACAA | R:CCTGGGGCATCACTTCTACC | NM_010548.2 |
| IL-1R | F:GAAGGTCTTAGCTGGTGCG | R:TTCATATTCTCCTGGGCGTGC | NM_001123382.1 |
| IL6-Rα | F:TGAATGATGACCCCAGGCAC | R:ACACCCATCCGCTCTCTACT | NM_001310676.1 |
| IL-10Rα | F:GCCAAGCCCTTCCTATGTGT | R:CCAGGGTGAACGTTGTGAGA | NM_001324486.1 |
| c-myb | F:GAGAGGGCCATGGGACTAGA | R:GACGTCAGCAAAGAGGGAGG | NM_001198914.1 |
| MMP9 | F: GCCGACTTTTGTGGTCTTCC | R: CTTCTCTCCCATCATCTGGGC | NM_013599.4 |
| Col1a1 | F: CGACCTCAAGATGTGCCACT | R: CCATCGGTCATGCTCTCTCC | NM_007742.4 |
| CCR2 | F: TTCTGGGCTCACTATGCTGC | R: GGCCACAGGTGTAATGGTGA | NM_009915.2 |
| TLR2 | F: ATGTTGAAGTCCAGCAGAAT | R: CCGAACCAGGAGGAAGAT | NM_011905.3 |
| TLR4 | F: TTCACCTCTGCCTTCACT | R: GGACTTCTCAACCTTCTCAA | NM_021297.3 |
| SCD1 | F: TGCCTCTTAGCCACTGAA | R: CTGTTGAGATGTGAGACTGT | NM_009127.4 |
| PPAR-γ | F: CCACCAACTTCGGAATCA | R: GCTCTTGTGAATGGAATGTC | NM_001127330.2 |
| PPARα | F:TTCCGTGTCAGTCTGCATCT | R:CCTGAAAGGTCCTAGCACCA | NM_011144.6 |
| PGC1α | F:TTGACTGGCGTCATTCGGG | R:TCGCAGGCTCATTGTTGTACT | NM_008904.2 |
| LDLR | F:CCAATCGACTCACGGGTTCA | R:CTCACACCAGTTCACCCCTC | NM_010700.3 |
| GR | F:TGAAGCTTCGGGATGCCATT | R:ATTGTGCTGTCCTTCCACTG | NM_008173.3 |
| RPS17 | F: GGAGATCGCCATTATCCCCA | R: ATCTCCTTGGTGTCGGGATC | NM_009092.3 |